projects/genomics/yeast/contents/quality/4R013-Y-E-1-Sequences

4R013-L2-P04-TGACCA-Sequences.txt FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content
Kmer Content

Basic Statistics

Measure	Value
Filename	4R013-L2-P04-TGACCA-Sequences.txt
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9530673
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA	244359	2.563921771316674	No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG	223770	2.347892955723064	No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA	207081	2.1727846501500996	No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG	185872	1.9502505227070532	No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC	49990	0.5245169989569467	No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT	40022	0.4199283723195623	No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG	36501	0.38298449647784577	No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG	24647	0.2586071308920157	No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG	23991	0.25172409125777373	No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG	22380	0.23482077288770686	No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG	21921	0.23000474363143084	No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT	19564	0.2052740661651071	No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA	17314	0.18166607961473447	No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA	16744	0.17568538968864003	No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC	15464	0.1622550684510947	No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT	15174	0.15921226129571334	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	14295	0.14998940788336773	TruSeq Adapter, Index 4 (100% over 50bp)
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA	13013	0.13653810176888873	No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA	12449	0.13062036647359532	No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC	11380	0.11940394975255159	No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT	11281	0.11836519834433519	No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG	10917	0.11454595074240823	No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC	10792	0.11323439593405417	No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG	10162	0.10662415969994983	No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC	9901	0.10388563326010662	No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT	9707	0.10185010019754114	No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT	9707	0.10185010019754114	No Hit
GCTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATC	9681	0.1015772967974035	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGGATCT	23430	76.86593	1
GGATCTC	25135	71.44676	2
GATCTCT	28700	62.60435	3
GACGCTC	9930	61.15017	2
GCGCCCC	32890	58.666996	94
ACGCTCA	10970	55.952393	3
CGACGCT	4275	53.76854	1
GCTCAAA	46925	52.00454	1
ATCTCTT	35225	50.967102	4
TCTCTTG	35280	50.92761	5
GCATTTC	39645	50.448936	94
CTCTTGG	37095	48.395252	6
CGCCGGG	715	45.07394	1
TCTTGGT	46205	38.994987	7
CGCATTT	62260	38.381386	94
CGCTCAA	16900	37.514812	4
CTCAAAC	67230	36.379654	2
CAGGCAT	67165	36.131737	8
ACAGGCA	67700	35.83232	7
AGGCATG	67840	35.765305	9