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4R013-L2-P10-TAGCTT-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P10-TAGCTT-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P10-TAGCTT-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25461649
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG12535514.923290710668425No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA11126234.36979945800054No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA10983214.313628704880819No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG8933423.5085787255962884No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC2130840.8368821673725846No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG2051780.8058315468884202No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT1794430.7047579675613311No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT1117780.4390053448619922No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG1096640.4307026618739423No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG1056850.415075237271553No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG979940.3848690239976209No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT727510.2857277625655746No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA720800.28309242657457107No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG650500.2554822745376782No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC607590.23862947761160322No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA578970.22738904302702467No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA531100.20858821830432114No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC517130.20310153517551044TruSeq Adapter, Index 10 (100% over 50bp)
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT477100.1873798511636069No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT465890.18297715124421046No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA465510.18282790717914618No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA458940.1802475558436926No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG458710.18015722390957475No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC430100.1689207168003926No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC425080.1669491241513855No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA397000.15592077323821407No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC396450.15570476209141051No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA390390.15332471200117478No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT371970.14609030232095335No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT348050.13669578117269623No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC341110.1339701132475748No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA326450.12821243431641052No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA306310.12030249886800341No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATC291580.11451732760906412No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG285780.11223939187913556No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC276130.10844937812158198No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT270810.106359961210682No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA256440.10071617906601414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT1087050.081.213981
GGATCTC1182050.074.5303652
CGACGCT154650.070.189091
GATCTCT1319600.066.786413
GACGCTC407550.065.766712
GCGCCCC1691900.064.5504194
ACGCTCA437250.061.718613
TCTCTTG1429400.061.2143865
ATCTCTT1444350.060.685384
GCTCAAA1915150.057.3861471
GCATTTC1928850.055.3343194
CTCTTGG1628250.053.77556
CTGCATG84400.048.4968228
CGCCGGG18350.047.2714651
TCTTGGT1899800.046.1128737
GCGCAGA49300.045.75429594
CTCAGAT108400.044.427151
CGCTCAA648850.042.4019784
AGACGCT104300.040.810891
CGTGCGT39500.039.8405231
Produced by FastQC (version 0.11.5)