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4R013-L2-P18-GTGAAA-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P18-GTGAAA-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P18-GTGAAA-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16607580
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG9453555.692310378754762No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA8385765.049356980366796No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA7548174.545014987132381No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG6884984.145685283466947No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG1659210.9990678955031378No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC1595290.9605794462528556No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT1306690.7868033753262064No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT923220.5559027865589087TruSeq Adapter, Index 19 (97% over 40bp)
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG838500.5048899358003996No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG793580.47784204562013255No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT785670.47307916023887886No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG714770.430387810867086No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT570210.34334322038490855No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA495570.2983998872803864No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG479650.28881390304908966No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC424650.2556964952148356No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA388540.23395341163492817No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC386280.23259258724028425No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA372820.22448785434121044No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT349550.21047618015388156No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG336300.2024978955392658No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT314810.18955802109639094No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA310270.18682432961334522No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT295060.17766586101045426No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA288630.17379413496728602No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA284320.1711989344624563No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC284230.1711447423405457No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA283840.17090990981226645No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC276510.16649626254999222No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC269690.16238970397854474No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA244910.14746880641249357No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA238040.1433321411066513No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT233810.14078511137685323No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC216330.13025979703243942No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT208650.12563540262940176No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG200490.12072198357617427No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG200420.12067983414802157No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATC199400.12006565676636813No Hit
GGGATACGTTGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAG197160.11871687506548215No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA194560.11715132487695379No Hit
CGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCC192840.11611565321377347No Hit
TGAGAATTCTGGCCGAGGAACAAATCCTTCCTCGCGGCTAGACACGGATT187170.11270154953340583No Hit
CAAATCCTTCCTCGCGGCTAGACACGGATTGCACGCCCTTTGGGCAAGGG167390.1007913254068323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT813400.082.298731
GGATCTC887900.075.2977452
CGACGCT103050.073.093211
GATCTCT973650.068.656563
GACGCTC284700.067.767182
GCGCCCC1259300.065.697894
ACGCTCA301350.064.350473
TCTCTTG1040300.063.768025
ATCTCTT1042600.063.6917954
GCTCAAA1307050.057.3921661
CTGCATG61200.057.1294868
GCATTTC1450550.055.6718494
CTCTTGG1197200.055.5077366
CTCAGAT75150.051.3448031
CGCCGGG13200.050.634471
TCTTGGT1390600.047.8485877
GCGCAGA31300.046.84403294
AGACGCT71850.045.9180641
CGCTCAA458900.042.8307574
TGCATGT83300.042.4240049
Produced by FastQC (version 0.11.5)