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4R013-L2-P13-AGTCAA-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P13-AGTCAA-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P13-AGTCAA-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17114418
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG3973072.321475378245407No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA3725122.1765975331442764No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG3354101.9598095594019032No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA3346111.9551409811306466No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC766600.4479264208692343No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG764170.4465065654000037No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT633370.37007977717968554No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG589580.3444931636004216No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT566080.3307620510379027No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC368570.21535643221989786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT350840.20499674601847404TruSeq Adapter, Index 13 (97% over 40bp)
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC321890.18808118394677517No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG316770.18508955431613275No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG313590.18323147185022592No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC262910.15361901292816382No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT256200.149698342064568No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA252730.1476708118266131No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA250920.14661322400796803No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG246530.14404813532075703No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT244230.14270423919761688No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG232710.13597307252867144No Hit
CTTTCTGGTGGGATGGGATACGTTGAGAATTCTGGCCGAGGAACAAATCC204030.119215272175776No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA195690.11434218797273737No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC189160.11052669158834383No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA188590.11019363907086996No Hit
CAAATCCTTCCTCGCGGCTAGACACGGATTGCACGCCCTTTGGGCAAGGG176800.10330471068312111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT422200.077.693281
GGATCTC458200.071.5340352
GATCTCT520450.063.041013
GCGCCCC544350.059.66303694
TCTCTTG609600.053.4969335
GCTCAAA690250.052.7954181
ATCTCTT622200.052.5723574
GACGCTC137200.051.7944872
CGACGCT56400.051.7647861
CTCTTGG661800.049.204156
CGCCGGG14850.048.192931
ACGCTCA153600.046.570193
GCATTTC745100.044.294694
TCTTGGT809500.040.6667
CGTGCGT22550.037.8319551
GCGCAGA31500.037.7444194
CAGGCAT1013900.035.6010488
GTTCTCG1101800.035.583155
CTCAAAC1032650.035.404342
AGGCATG1022650.035.2964449
Produced by FastQC (version 0.11.5)