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4R013-L2-P08-ACTTGA-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P08-ACTTGA-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P08-ACTTGA-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19453785
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA5246332.6968170975468273No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG5197412.6716703201973293No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA4315912.218545131448713No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG3526131.8125675800364813No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC820060.42154264581416934No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG747340.38416174538785125No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT597930.307359210559796No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG554280.2849214176058798No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG478060.2457413814329705No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG453810.23327594090301706No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT445390.22894773433550336No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG381970.19634739460727052No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC294920.15160031839562327No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT288940.14852636646287598No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA285490.1467529326555218No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA282340.1451337104835897No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA259480.13338278386442534No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT251770.12941954483407728No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC251590.12932701785282402No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC248830.12790827080694064No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT248130.1275484436576224No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT235280.12094304527370894No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA229400.1179204972194357No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA213110.10954680541601546No Hit
CTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAGG205880.10583030500234272No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA199110.10235026242965059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT442150.077.469261
GGATCTC471050.072.5170062
GATCTCT543900.062.950973
GACGCTC178700.059.229192
GCGCCCC765650.059.12524894
CGACGCT78500.057.478881
ACGCTCA195500.054.4759863
GCATTTC933600.053.52253394
TCTCTTG653400.052.1630255
ATCTCTT664000.051.5430454
GCTCAAA814650.051.413221
CTCTTGG696450.048.9953546
TCTTGGT847700.040.596087
GTTCTCG1269400.040.0490765
TCGCATC1290250.039.364869
CTCGCAT1296300.039.155768
TCTCGCA1304850.039.0720637
GGTTCTC1309350.038.978184
TTCTCGC1323900.038.5108346
CTCAAAC1340850.038.5042151
Produced by FastQC (version 0.11.5)