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4R013-L2-P06-GCCAAT-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P06-GCCAAT-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P06-GCCAAT-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18417804
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG11053136.001328931505624No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA10027555.444487301526284No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA9630675.229000156587615No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG8132354.415482975060437No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC1758200.9546197798608347No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG1660200.9014103961579786No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT1602920.8703100543365538No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT1212420.6582869488675197No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG1004340.5453093104910879No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG1001030.5435121364088792No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG995680.5406073384210192No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA653760.35496088458754366No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT645850.3506661271886703No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC645530.3504923822622936No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA529630.2875641417402422No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA521190.28298161930705745No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA517840.28116272710905166No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT515770.28003881461655256No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA515770.28003881461655256No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG505980.2747233057752162No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT485770.2637502277687394No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA472330.25645294086091913No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT456860.24805345957639685No Hit
TTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGC452570.24572419165715959No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG410980.22314278075714128No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA398290.2162527085205163No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG359460.19516984761049688No Hit
CAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGAT332770.1806784348448925No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGA311770.1692764240514233No Hit
AAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAGA309850.16823395449316325No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA296160.1608009293616112No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC294100.15968244639806137No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATC286440.15552342722291973No Hit
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAAT266610.14475667131651526No Hit
CTCAGATCTGCATGTGTTGTATAACACTGGCAAGAAAAGTTCCCGTTTGC260990.14170527604702493No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG260290.14132520902057596No Hit
AACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCA260080.14121118891264126No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA255560.13875704182757076No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT245440.13326235853090845No Hit
CGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCC244570.13278998951232188No Hit
TTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA234560.12735503103410156No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACT226330.1228865287088515No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC225670.12252817979819962No Hit
GTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCG204430.1109958603099479No Hit
CAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCAT198880.10798247174310248No Hit
CTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATCAAATAAACG195370.10607670708190835No Hit
ATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATT192240.10437726452078651No Hit
GGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG191400.10392118408904774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT969600.082.0802461
GGATCTC1048950.075.804662
CGACGCT141500.073.78821
GACGCTC361600.071.15932
GATCTCT1153850.068.876183
ACGCTCA378750.068.259613
TCTCTTG1241400.063.555775
ATCTCTT1261700.062.6457864
GCGCCCC1537300.059.97664394
CTCTTGG1404000.056.215936
CGGGCGC9100.055.727721
CGCCGGG10950.054.969081
CTGCATG48150.054.557868
GCTCAAA1757700.054.1787381
GCGCAGA39550.053.7073594
GCATTTC1843850.052.80620694
GGACGCT39250.051.1982421
AGACGCT79500.050.3157771
CGTGCGT33800.048.9370271
TCTTGGT1631800.048.474197
Produced by FastQC (version 0.11.5)