Worg
4R013-L2-P03-TTAGGC-Sequences.txt FastQC Report
FastQCFastQC Report
Tue 14 Feb 2017
4R013-L2-P03-TTAGGC-Sequences.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R013-L2-P03-TTAGGC-Sequences.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518641
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG2629703.0869947448190387No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA2207432.591293611269685No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA2158692.5340779121928017No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACG1831362.1498264805383864No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC437270.5133095760227482No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG425170.4991054324275433No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT365050.4285307949941781No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC229040.26886917760708545TruSeq Adapter, Index 3 (100% over 50bp)
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT217610.2554515444423588No Hit
CGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG214360.25163638190645665No Hit
CAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAAG214270.2515307312516163No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG205450.24117696707726036No Hit
CGCAAAGATCGATTTATTACAGCCGGGACACTTCAGAACGGACTCTCCCG176690.20741571337493858No Hit
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTA156580.18380866149894098No Hit
GGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT154850.18177782113367613No Hit
GCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCAC129190.15165564554252256No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG126530.14853308174390728No Hit
ATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAA122740.14408401527896295No Hit
CCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAA122090.1433209827717825No Hit
CCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC118100.13863713707385955No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA115990.13616021616593538No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGT115710.13583152523976535No Hit
AACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATA113730.13350721083327727No Hit
CATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAAT104090.12219085180370907No Hit
GAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA103560.1215686868363158No Hit
GTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATT100250.11768309053052006No Hit
CACGGATTGCACGCCCTTTGGGCAAGGGATAGTTCTCTATTCCGCACCGT95810.11247099155839528No Hit
CAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGA92260.10830366017302526No Hit
CGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCC90670.10643716527084543No Hit
AGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG87390.10258678584999649No Hit
CTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCA85250.10007464805712554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCT233100.078.8082661
GGATCTC253750.072.161452
GATCTCT279850.065.431373
GCGCCCC369850.062.5148894
GACGCTC88300.061.5303652
ACGCTCA94300.057.8645943
TCTCTTG336900.054.1836175
ATCTCTT339450.053.7212074
GCATTTC407450.052.3977294
CGACGCT42250.052.0501021
CTCTTGG363300.050.266816
GCTCAAA427050.048.676591
CGCCGGG6250.041.7073861
TCTTGGT444900.041.2686127
GTTCTCG662200.039.482965
CGCTCAA140950.039.37994
TCGCATC669450.039.113969
GGTTCTC676300.038.805734
CTCGCAT681550.038.4471248
TCTCGCA682700.038.4443177
Produced by FastQC (version 0.11.5)