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26688_ID1144_8_S62_L007-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26688_ID1144_8_S62_L007-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26688_ID1144_8_S62_L007-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6452598
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC75390.1168366602103525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC53600.045.7025641
TCTTCCG82450.029.2896778
GTGTGCT91950.026.7045882
ATTACGG24350.026.08556740-41
CTTCCGA88200.026.0034319
GTGCTCT95350.025.9347634
TGTGCTC100750.024.1438453
TTACGGC24900.022.72706842-43
CCCCCGT18900.022.6743551
TCCGATC51250.022.31658710-11
CCGATCT54350.020.77477512-13
TTCCGAT58500.019.84708210-11
CGTAACG7500.019.63294840-41
AATTACG29300.019.0180640-41
TGCTCTT130100.018.9174755
GGGGGGT101300.018.0651951
CGGAATA26600.017.8528424
GTCGGAA36100.017.83622226-27
CGGGCTA11450.017.7022131
TAATTAC37900.017.67361338-39
Produced by FastQC (version 0.11.5)