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26687_ID1144_7_S54_L006-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26687_ID1144_7_S54_L006-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26687_ID1144_7_S54_L006-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803698
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC41250.10844709543186656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC33650.046.7091521
TCTTCCG51750.030.0639638
GTGTGCT54300.029.4772282
GTGCTCT55900.028.2189184
CTTCCGA55900.027.007379
TGTGCTC58900.026.881853
GCGGAAT14650.025.6389143
ATTACGG13750.025.34593240-41
CGGAATA14800.023.8153864
CCCCCGT10550.022.4568861
TCCGATC35100.021.66903910-11
TTACGGC13350.021.35808842-43
TGCTCTT76150.020.8652525
CCGATCT37350.020.66908512-13
CGATCTA6700.020.20691912-13
GGCGGAA21950.020.0071742
TTCCGAT39500.019.47408110-11
CGGGGGT28550.018.6208671
TGCGTAT16550.018.4531579
CGGGGGG35200.018.2221011
Produced by FastQC (version 0.11.5)