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26685_ID1144_5_S13_L004-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26685_ID1144_5_S13_L004-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26685_ID1144_5_S13_L004-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18544266
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC203580.10978056505444864No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187420.10106628108117087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG66850.025.52271340-41
TCCGATC105900.025.3290966
CCGATCT112300.023.8248837
TTACGGC64400.022.28806342-43
TTCCGAT122850.021.8672165
CGATCTA21250.021.435688
CGTGTGC129450.020.8419131
CCCCCGT50200.019.909721
CGGAATA75650.019.3208474
GCGGAAT78800.019.0623723
CGGGGGA116850.018.1945721
CGGGGTG154250.018.127711
CGGGGGG170550.018.0889051
GGGGGGT329800.018.0605431
GTCGGAA97700.017.87714426-27
AATTACG79650.017.76651640-41
CGGGGGT138200.017.5575541
GGGGGTA177650.017.4635221
GTGTCGG104000.017.34867524-25
CGTAACG22600.017.21598440-41
Produced by FastQC (version 0.11.5)