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26684_ID1144_4_S51_L006-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26684_ID1144_4_S51_L006-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26684_ID1144_4_S51_L006-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613292
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC36240.10029635025345308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC23150.027.8501191
ATTACGG13250.025.11962140-41
CGGAATA12850.023.956084
TTACGGC12400.023.57337642-43
CGGGCTA6350.021.9529321
TCCGATC21900.021.7000316
CCCCCGT10200.021.6390631
GCGGAAT13300.020.9634513
CCGATCT23600.020.3728627
CGATCTA4405.456968E-1219.7447818
TTCCGAT25550.018.6393075
GGGGGTA31800.018.4480021
GGTTCGA10250.018.1329774
CGTAACG4050.017.86553840-41
AATTACG16400.017.64766740-41
TAATTAC19900.017.45268838-39
GGCAAGT35650.017.4328024
CTATACT20650.017.43145468-69
GTTCGAT10400.017.311525
CGGGGGT23850.017.2911971
Produced by FastQC (version 0.11.5)