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26683_ID1144_3_S72_L008-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26683_ID1144_3_S72_L008-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26683_ID1144_3_S72_L008-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4051085
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55060.13591420570044815No Hit
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC46460.11468532504255033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC28700.037.156311
ATTACGG14700.027.9152440-41
TCCGATC33500.025.7240856
TTACGGC14400.025.08506242-43
CCGATCT35450.023.6474157
TTCCGAT38000.022.1141515
CGATCTA6050.021.9758328
CGTAACG4150.021.58658640-41
TCTTCCG54100.021.1563518
CCCCCGT11000.021.0748231
GTGTGCT54600.020.2741532
GCGGAAT15800.020.1747253
GTGCTCT55550.019.7182774
AATTACG18550.019.47316240-41
TGTGCTC56000.019.3533253
GGCAAGT36850.019.3445594
CGGAATA16650.019.1443084
TAATTAC22500.019.13665638-39
CTTCCGA56550.019.0133469
GTCGGAA21800.018.95871726-27
Produced by FastQC (version 0.11.5)