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26682_ID1144_2_S10_L004-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26682_ID1144_2_S10_L004-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26682_ID1144_2_S10_L004-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17064085
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC201220.1179201814805775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC129800.030.1379781
ATTACGG62900.027.18918240-41
CGGAATA68950.023.5203444
GCGGAAT71900.022.8792363
TTACGGC64850.022.66257942-43
CCCCCGT46500.022.3755571
TCCGATC109300.021.978066
TCTTCCG186200.020.4317268
CCGATCT117900.020.1691657
CGTAACG20250.020.04000540-41
AATTACG77150.018.74819240-41
CTTCCGA200450.018.5736989
GTCGGAA91550.018.49134326-27
TTCCGAT130050.018.4146925
CGGGGGG153550.017.9432981
GGGGGTA162350.017.9373871
CGGGGGA111500.017.8810481
GGCAAGT167850.017.6959274
GTGTGCT226200.017.6795882
CTATACT97300.017.6242968-69
Produced by FastQC (version 0.11.5)