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26681_ID1144_1_S9_L004-R2_001.fastq.gz FastQC Report
FastQCFastQC Report
Sat 1 Dec 2018
26681_ID1144_1_S9_L004-R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename26681_ID1144_1_S9_L004-R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15873256
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTC179760.11324708679807093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC136150.046.599051
TCTTCCG223500.028.0349458
GTGCTCT228650.027.9751824
GTGTGCT237700.027.1059362
TGTGCTC240300.026.571873
ATTACGG59000.026.29611640-41
CTTCCGA243600.025.0569239
TCCGATC152250.024.7160157
CCGATCT156150.024.061318
TTCCGAT174700.021.7479866
TTACGGC60600.021.12091642-43
TGCTCTT320550.020.1177145
CGATCTA25700.018.8927028
CGTAACG20750.018.37913940-41
GTCGGAA86150.018.07824526-27
CGGAATA63000.017.9336684
CCCCCGT43350.017.9100281
GCGGAAT63900.017.5005113
GTGTCGG91400.017.38810524-25
CGGGGGA102350.017.1528151
Produced by FastQC (version 0.11.5)