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63287_ACTGAT_S42_L004_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63287_ACTGAT_S42_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63287_ACTGAT_S42_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12084923
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14800.020.51528446
TCGTATG14600.020.48104945
CTCGTAT17450.017.7950144
TATCTCG17800.017.18687641
TCTCGTA20700.015.22328443
GATCGGA21600.012.5711541
ATCTCGT24150.012.47726742
CGGGGAT15900.010.7098021
ACACGTC29700.010.53132613
ATATATC31200.010.24712838
CGTCTGA31850.09.676002516
CGCGGTT6450.09.6327641
GATATAT32800.09.396646537
ACGTCTG32700.09.35415415
ATCGGAA30150.09.0785022
TATATCT34800.09.055078539
CTCGGGT18650.09.0072221
TGATATA35600.08.91613136
CGGAGAT15700.08.7942431
CTCGGAT18150.08.7482121
Produced by FastQC (version 0.11.5)