63286_GATCAG_S41_L004_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63286_GATCAG_S41_L004_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63286_GATCAG_S41_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10071435
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT1400.00445080611.505141
CCGGGAT15450.011.3189731
CGACCTA3153.3183824E-810.9515094
CGGAAAT9400.010.5259791
CTCGAAT11050.010.4118911
CGCGAAC1550.00948617910.3917391
CTCGGAT15200.010.2940721
CGGCAAT8000.09.491741
CGAGAAT11650.09.4806311
CGCGGAT6001.8189894E-129.2041121
TCGTATC3754.2240026E-79.2002282
CTGGAAT58100.09.0298521
CGGGTAT6750.08.8632191
CCGGAAT10650.08.85841751
CGATCGT2350.0022208938.807814
CGCATAT3451.3510624E-58.670541
CGGGAAT12300.08.6054711
CGCGTCT5402.066372E-98.52232551
CGCGGGT9200.08.50379851
TCGGGTC11700.08.45322