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63258_ATGTCA_S13_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63258_ATGTCA_S13_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63258_ATGTCA_S13_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8235028
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT6250.011.0459681
CGACAAT4506.548362E-1110.2277491
CGGTCTA2605.2613002E-59.73094544
CTCAGAT35300.09.7134641
CTCGGAT12200.09.4313261
CGCGGAT4903.2923708E-109.3928291
CGGAAAT8400.09.3145561
CCCGTAT4701.540684E-99.3028971
CGAAGAT14800.09.1738761
CCGAGAT17300.09.0453491
TATATCG2801.1825963E-49.03560435
AATCGAC2801.1832999E-49.03510955
AGATCGA11800.08.9655024
CGCGACT3602.386665E-68.9492791
CTCGTTT9350.08.8604021
GATCGAA6750.08.8586665
CGAGAAT8450.08.7147681
CGATCCA6900.08.6660874
AATCGGC5055.673428E-98.6528865
GTCGGTT5901.382432E-108.5809071
Produced by FastQC (version 0.11.5)