63258_ATGTCA_S13_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63258_ATGTCA_S13_L002_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63258_ATGTCA_S13_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8207513
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA2251.0681777E-511.2436764
GTCGGTT6000.011.1222761
CTCGGAT11700.011.0141031
CGCGAGT3204.146932E-810.7866911
CGGGGAT11050.09.9959921
GTATCGA4151.9119398E-89.4210424
CGGCAAT6850.09.4062041
CGAAGAT14950.09.3893511
CCGGGAT10800.09.3750981
ATCGAAG11550.09.1595476
CGACGAT4558.718416E-99.1034921
TCGTATG3805.1101415E-79.07982145
CTCGGGT12200.09.0537461
CTCGCAT9950.09.0196251
CTCGAAT9050.08.8995161
CTCGCGT3652.8778413E-68.8263691
CGGGAAT9700.08.7776231
CGATAAC4507.0493115E-88.6932731
CGTATCG3451.3595483E-58.666183
CCCGAAT8550.08.6125311