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63257_GCCAAT_S12_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Tue 21 Mar 2017
63257_GCCAAT_S12_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63257_GCCAAT_S12_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9907094
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2601.2551027E-1014.1623521
CGGCAAT8000.011.5069121
GTCGGTT6850.010.7509831
CTCGAAT12750.010.4690331
CTCGGGT15100.010.3638411
TAGCGCG2056.3713454E-410.09740818
CCCGTAT5500.010.0423961
CCGGAAT10400.09.7366171
GTCGCAT5950.09.6696731
CGAGAAT9050.09.6632631
CGCGAAC2250.00148595679.2055291
CTCGTAT7500.09.2055281
CTCGGTT9050.09.1546711
CGGGAAT11700.09.0481691
TCGAATC7350.08.7623022
CGGAGAT15500.08.7600991
CGGAAAT11850.08.7394271
CCGGCAT12400.08.7229811
CGCGTTA2450.0032362058.44927932
TCGCATT8450.08.4382532
Produced by FastQC (version 0.11.5)