63255_GTCCGC_S10_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63255_GTCCGC_S10_L003_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63255_GTCCGC_S10_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10483274
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT1402.9829447E-413.1511171
CTCGGTT10500.011.3976351
CGCGTAT1500.007428616310.7400791
CGGAGAT14550.010.2813731
CTCGCAT10700.010.1091541
TATGCGC3002.3038865E-69.9661297
CGACAAT6500.09.9139191
CTCGGGT17450.09.7597121
CGGCAAT9100.09.3575261
GTTCGAT7150.09.3345351
CTCGGAT15300.09.326121
CCCGTAT6001.8189894E-129.2057821
TCGCATT9850.09.1073682
CGCATAT4303.369132E-89.0987391
CGCAAAT8150.09.036351
CTCAAAT40250.09.0342461
CGCGGAT6650.08.9981341
GGAATCG10600.08.8961723
CGGGAAT12500.08.8375511
GTCGCAT7050.08.8140481