63255_GTCCGC_S10_L002_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63255_GTCCGC_S10_L002_R1_001.fastq.gz

[OK]Basic Statistics

MeasureValue
Filename63255_GTCCGC_S10_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10437362
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC2755.9355443E-811.7142411
TTACGCG1450.00580070411.103435524
TACGCGA1450.005801531511.10322325
CGCCGAT7000.010.8476261
CGGAAAT11550.010.5587541
CGGGAAT10550.010.4690571
CGAAGAT17750.010.2411211
CTCGAAT12950.010.1280041
CGCGGAT6200.010.02053451
CTCGGAT15000.09.3574481
CTCGCAT9400.09.3019621
CCCGTAT5801.0913936E-119.1247011
CTCGTTT11300.08.9596911
CTCGAAC14400.08.9483791
CCGGGAT13700.08.9017241
CGTATCG4151.9178333E-78.8671213
CTCGAGT11250.08.7949781
CTGGAAT61650.08.47251
CTCGGTT9000.08.4370431
GTCGCAT6052.3283064E-108.3673141