Worg
63247_CAGATC_S2_L003_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 20 Mar 2017
63247_CAGATC_S2_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename63247_CAGATC_S2_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11898351
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT7750.011.58027651
CTCGGTT10350.010.0052561
CTCGACT11500.010.0052561
CTCGGAT16550.09.8722551
CCGGAAT12600.09.3144171
CGAGAAT10700.09.24784851
CGCGGAT8750.09.2048351
CAATCGA4051.3202225E-79.0858474
AATCGAA10200.09.0190395
CTCGGGT17250.08.9380291
CTCAGAT45900.08.9240781
CTCGAAC14900.08.8032821
CTCGAAT13600.08.7987391
CGCATAT4455.883703E-88.7911361
GTCGAAT5004.6966306E-98.7445941
CGAAAAT11950.08.6656411
CCGACAT11200.08.6295331
TTCGATC7300.08.5075282
GTCGAGT4953.6672645E-88.36803151
GAATCGA8300.08.312734
Produced by FastQC (version 0.11.5)