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33730_GATCAG_L008_R1_001.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACA2206.0856655E-712.5446712
CCGGGAT6700.011.6880051
CCCGTAT2208.183466E-611.5161241
GCGCTTA1903.1609464E-410.89419328
CTCGCAT4609.094947E-1210.5147221
CCGTAAT2056.287153E-410.1117181
ATAGCCG2352.0420265E-49.78796921
CGCCAAT3103.4355853E-69.6586851
TCGCATT3451.3497101E-69.3327492
CGTACTA2000.00588661349.19965635
TCGGCTA2503.8061143E-49.1994254
CGCGGGT3307.6060496E-69.073311
CGCAAAT3052.9721037E-59.0618681
CGGTCGG2100.0087864068.7613574
CGCTTAT2656.8081653E-48.67881229
TAATCGG2450.00323783688.44855726
CGGACTA2550.00464444568.11734313
CGGAATC3753.869961E-57.9728352
GGCCGAT4051.1502016E-57.96176431
GTTCAAT6458.403731E-107.85596041
Produced by FastQC (version 0.11.5)