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33730_GATCAG_L007_R1_003.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L007_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L007_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA1401.8158144E-514.7843442
ATTCGAC1352.2054055E-413.6283665
ACCGGTA1402.9998267E-413.14196945
TACCGGT1402.9998267E-413.14196944
CGCGAAT1556.974697E-411.8899311
GTCGGTT3203.3160177E-911.5183721
GTACGAT1500.007465746310.7326079
CTCGCAT4101.2551027E-1010.6755641
CGCATAG1800.002465830710.2212753
CCCGTAT2301.6190021E-410.0159751
GTCGCAT3307.1980503E-79.7731641
CGGGCAT7550.09.4587951
CGCGGAT2707.813209E-59.385341
CGGAAAT5251.2187229E-109.2146981
GTTCGAT2503.743424E-49.2146971
CCGCAAT3002.4402627E-59.2146971
GATCGAC2000.00588928639.1991475
GCGAATT2503.8072647E-49.1991472
CGCTGAT4051.2858436E-79.1009351
AATCGGA2554.6411532E-49.0187725
Produced by FastQC (version 0.11.5)