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33730_GATCAG_L006_R1_004.fastq.gz FastQC Report
FastQCFastQC Report
Mon 22 May 2017
33730_GATCAG_L006_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename33730_GATCAG_L006_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416102
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAC706.026396E-419.713093
CGCGAGA1055.0660316E-415.3508321
CTCGGAT1502.0243497E-615.3508321
CGGTTGC900.003244849915.3324033
CGCGGAT1606.30132E-512.9522641
CCGGAAT1556.996521E-411.8845161
CGGGCAT3305.3951226E-911.1642421
TGTTGCG1450.005796027411.103559523
CGGAGAT2301.6247785E-410.0114121
GACTTAA1900.0038549789.6836234
GAGATCT4051.27929525E-89.6537353
GTTTACT2300.00182425678.9994546
CTCGGCA2350.0021940098.81856251
TCGGGTC2100.0087804638.76137352
GTGAGAT2708.0941524E-48.528241
AACCTAA2708.191327E-48.5180025
CTCGGCT3804.901014E-68.4833551
CCCGAGT2450.00320054968.4586221
GATTCAA2800.00117470728.2137885
CCGGGTT3451.2589431E-48.009131
Produced by FastQC (version 0.11.5)