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4R023-L2-P10-ACTGAT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P10-ACTGAT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P10-ACTGAT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44530595
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG105850.017.1701452
CTCGAAT215500.015.7588241
CGAATGT128700.014.6677173
ACGATAC64550.013.015532560-61
CCGCTAT37550.011.9755211
CTCAAAT359100.011.870231
CTCCGAT143750.011.4375331
CTCGTAT108450.011.4026941
CGATAAC56300.011.3984371
CTCGTTT183150.011.381151
CTCGATT67050.011.2476081
GTCGGTT105350.011.1602091
CGCGTAA10850.010.793031
GTTCGAT79150.010.7709391
GTCGTTT140650.010.5905971
CGATTAG74500.010.4371274
CGCTAGT21600.010.1924181
CATACGA75450.010.18805356-57
CTCGGAT208100.010.0841441
GCATACG83150.010.06195956-57
Produced by FastQC (version 0.11.5)