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4R023-L2-P12-GATCAG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P12-GATCAG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P12-GATCAG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32548922
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG68400.014.2421662
CTCGAAT141600.013.3624751
GTCGGTT69650.011.5658121
CGAATGT84100.011.360683
CTCCGAT105700.011.2988421
CTCGTTT127750.011.1816961
CTCGTAT85700.010.7113461
CCGCTAT24500.010.70511
CTCGTCT150500.010.3939061
ACGATAC41200.010.13832260-61
TCGGTTT92250.010.103122
CCCGAAT70300.010.0598241
CTCAAAT256500.010.0060341
CGATTAG53500.09.9796934
CTCGATT50850.09.9472171
GTCGTTT101650.09.9060341
CCCGTAT43500.09.6899611
TCGTTTT162500.09.5398912
CCCGTTT80250.09.279421
CTCAGTT239050.09.149491
Produced by FastQC (version 0.11.5)