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4R023-L2-P05-CAGATC-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L2-P05-CAGATC-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P05-CAGATC-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51031583
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG114750.013.9201652
CTCGAAT240050.013.1717891
CTCGTTT191950.012.2750311
CGAATGT131150.012.0009783
CTCCGAT165050.010.9550531
CTCGTAT127250.010.9330511
GTCGGTT119200.010.806851
ACGATAC66300.010.51343460-61
CTCAAAT400150.010.3600811
CCCGTTT132150.010.2795371
CGATTAG80800.010.2613534
GTCGTTT162000.010.1203551
TCGTTTT250850.09.9530422
CTCGGTT163100.09.9084991
GTTCGAT93000.09.7715061
TCGGTTT147800.09.7615552
CCCGAAT107650.09.74712851
CTCCGTT176300.09.5651751
CTCGTCT246200.09.3799721
CTCCTAT172100.09.3631131
Produced by FastQC (version 0.11.5)