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4R023-L2-P04-GCCAAT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Fri 14 Apr 2017
4R023-L2-P04-GCCAAT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P04-GCCAAT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37916599
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT29700.013.4058581
TCGAATG82650.011.8450152
CTCGTTT145000.011.6619541
CTCCGAT122650.011.648351
CTCGAAT177400.011.3011421
GTCGTTT121250.010.81703951
CTCAAAT297700.010.5421241
CCCGAAT81400.010.1854691
CCCGTTT96550.010.1397251
GTCGGTT87300.010.0336631
CCCGTAT48500.09.85123351
TCGTTTT186700.09.7597212
CGATTAG59550.09.7536964
CTCGGAT193600.09.6054411
CTCGTAT99650.09.448241
CCCGGTT91700.09.2968151
CTCGATT59650.09.2662441
CGAATGT98500.09.130593
CGCCTAT22650.09.0994811
TATGCCG40500.09.0194546-47
Produced by FastQC (version 0.11.5)