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4R023-L2-P03-ACAGTG-Sequences.txt.gz FastQC Report
FastQCFastQC Report
Fri 14 Apr 2017
4R023-L2-P03-ACAGTG-Sequences.txt.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L2-P03-ACAGTG-Sequences.txt.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40559619
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG95950.018.4668272
CTCGAAT186550.015.6713471
TATGCCG49300.015.63194544-45
CGAATGT106800.015.4466163
TCGTATG70600.012.74599542-43
CTCCGAT124250.012.5666731
ACGATAC52800.012.550158560-61
CTCGATT62750.012.3280591
CTCAAAT326450.012.28459551
CGATTAG67100.012.1175164
CTCGTTT158200.011.96981051
CGCGTAA11000.011.6490881
GTCGGTT91050.010.6333731
CGATACT63800.010.4660-61
CCCGTAT53900.010.3899641
TACGATA63300.010.28266158-59
CCCGAAT81250.010.2804031
CATACGA64150.010.25582956-57
CTCAATT187700.010.2402731
TCGGTTT113500.010.22814752
Produced by FastQC (version 0.11.5)