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4R019-L5-P5-CAGATC-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R019-L5-P5-CAGATC-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P5-CAGATC-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42455637
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG56100.014.77311382-83
CCCGAAT81400.014.1378151
CCCGTAT56400.014.1007241
TCGACCG61900.013.71617730-31
GTTCGAC64850.013.12829928-29
GTCCGTT69350.012.6144051
CTCCGTT149850.012.1128561
CTCCGAT121250.011.9219791
TATGCCG55400.011.73687844-45
GTCCGGT58000.011.6953071
CGACCGT61200.011.57997832-33
GTCGTTT127250.011.5436571
CTCCTAT160700.011.4988081
GTCGGTT90350.011.4946681
CCCGGTT92450.011.2335661
CTCGTTT162300.010.98191451
CTCCGGT163250.010.7460711
CCCGGAT156700.010.4787561
AAGTTCG86100.010.34997726-27
CGCTAAT45450.010.1895623
Produced by FastQC (version 0.11.5)