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4R019-L5-P2-CGATGT-Sequences.txt.gz FastQC Report
FastQCFastQC Report
Fri 14 Apr 2017
4R019-L5-P2-CGATGT-Sequences.txt.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4R019-L5-P2-CGATGT-Sequences.txt.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74220474
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT313850.013.718331
TCGAATG161150.013.5245072
CTCGTTT279100.011.9065921
CTCCGAT233050.011.7583771
CGAATGT186600.011.2012913
CGCGTAA22500.011.0718771
CCGCTAT57450.010.9633341
CTCAAAT585900.010.8302431
GTCGGTT162000.010.590241
CGATTAG125400.010.2254794
CTCGTAT181800.010.1607691
ACGATAC87650.010.04823760-61
TCGTTTT355000.09.7779962
CTCGATT115550.09.6813291
CCGGCAT194150.09.5870661
CTCGGTT249350.09.5382591
CTCGGAT386200.09.5174891
CTCGTCT355400.09.3900711
CTCATTT664600.09.3850911
TCGGTTT209750.09.2935132
Produced by FastQC (version 0.11.5)