4R023-L4-P8-GTGAAA-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P8-GTGAAA-Sequences.txt.gz_trimmed.txt

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P8-GTGAAA-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41757236
Sequences flagged as poor quality0
Sequence length60-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA30850.016.55471
CTCGAAT149400.014.4263761
TCGACCG49100.013.54634626-27
CGCGTTT21100.012.4352811
CTCGATT73100.012.3705781
CGATCCG54450.012.32508278-79
TCGAATG91500.012.1360482
CTCGTTT154400.012.0170521
CTCAAAT330700.011.7454691
CTCCGAT124550.011.3985321
CGAATGT95300.011.1113183
GTTCGAC61150.011.1067624-25
CTCGCGT21500.010.6784361
CTCATTT385750.010.5672831
CGCTATT34200.010.5490811
GCGTAAC49000.010.4227062
CGACCGT53750.010.28296528-29
GTCGGTT77600.010.1437281
CTGGATT343500.010.1210881
CGCATAT30700.010.0729251