4R023-L4-P5-CAGATC-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P5-CAGATC-Sequences.txt.gz_trimmed.txt

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P5-CAGATC-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42785289
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT166750.011.9159221
CGCGTAA7903.6379788E-1211.8639861
CTCGTTT157550.011.570681
CTCGTAT100500.011.5641391
CTCCGAT140000.011.3140051
TCGAATG88100.011.0108822
CTCGATT76750.010.7463741
CTCAAAT316850.010.6637331
CGCATAT33400.010.1021481
CCGGGAT110900.010.0993641
GTCGGTT83300.010.0138871
CGATTAG68200.09.825994
CCCGAAT88350.09.7597561
CTCGGAT210500.09.7064871
CGAATGT99350.09.6697153
CTCGGTT138650.09.4638071
CCGCTAT26250.09.4618111
CCGGTTT99400.09.3819771
CTCATTT389750.09.3424881
CCGGTAT60200.09.341411