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4R023-L4-P3-ACAGTG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L4-P3-ACAGTG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L4-P3-ACAGTG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37732984
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG96500.026.0135244-45
TCGTATG117500.024.49307442-43
CGTATGC116950.021.54474642-43
ATGCCGT158450.018.5920646-47
GTATGCC166400.017.70349344-45
CTCGTAT144200.017.42432840-41
GCCGTCT172950.016.8843348-49
CTCGAAT143600.015.3333841
TCTCGTA167150.015.17202840-41
TCGAATG78400.014.5803632
TGCCGTC183950.013.99062146-47
CGCGTTT20850.013.4815471
GATCTCG208750.012.35064338-39
CGCATAT27550.011.7333511
CTCGTTT138800.011.7120941
CCGTCTT223600.011.67726248-49
CGAATGT87650.011.59851553
ATCTCGT194950.011.41114138-39
CTCCGAT117750.011.3788841
CTCAAAT298500.011.3785841
Produced by FastQC (version 0.11.5)