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4R023-L3-P05-CAGATC-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L3-P05-CAGATC-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L3-P05-CAGATC-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39653302
Sequences flagged as poor quality0
Sequence length60-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT146400.013.8227221
TCGAATG84150.013.5827162
CTCGTTT144950.012.7975811
CGCGTAA8950.012.5614321
CTCAAAT320200.011.4695311
CGAATGT95100.011.2799023
TCGTATG71950.010.93544942-43
GTCGGTT72550.010.7181941
CTCGATT69950.010.6478111
CGCTATT24000.010.5398261
TATGCCG54550.010.47427644-45
CTCCGAT123850.010.4391231
CGCGATT12150.010.4097041
ACGATAC42900.09.95750360-61
CCGGCAT89050.09.8369081
CGCATAT34150.09.7390951
CTCATTT363250.09.6975761
CTCAATT202800.09.5627611
CTGGATT333500.09.5091941
CTCGGAT187650.09.3612451
Produced by FastQC (version 0.11.5)