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4R023-L3-P04-GCCAAT-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L3-P04-GCCAAT-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L3-P04-GCCAAT-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39061868
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT148050.013.5119441
TCGTATG86400.013.03728442-43
CTCGTTT142100.012.2973951
TATGCCG64150.011.86429644-45
CTCAAAT294450.011.3919961
TCGAATG83700.011.3623862
GTCGGTT74850.011.2036511
CTCCGAT127000.011.0666431
CGCATAT30200.010.7038631
TATCTCG103650.010.12191938-39
CTCGATT71550.010.017981
CGAATGT89900.09.9013143
CTCATTT341050.09.753011
CTCGTCT179450.09.7117581
CTCGGAT182500.09.7034751
CGCGTTT20750.09.4826471
CTCATAT164450.09.315631
CTCAGTT282300.09.2768241
TCGGTTT108850.09.2535622
CGATTAG72050.09.2332224
Produced by FastQC (version 0.11.5)