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4R023-L3-P03-ACAGTG-Sequences.txt.gz_trimmed.txt FastQC Report
FastQCFastQC Report
Sat 15 Apr 2017
4R023-L3-P03-ACAGTG-Sequences.txt.gz_trimmed.txt

Summary

[OK]Basic Statistics

MeasureValue
Filename4R023-L3-P03-ACAGTG-Sequences.txt.gz_trimmed.txt
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38373659
Sequences flagged as poor quality0
Sequence length60-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT145450.015.0407891
TATGCCG49650.014.00798344-45
TCGAATG81300.013.4255912
CTCGTTT144200.013.254471
TCGTATG66800.012.40979642-43
CTCCGAT124600.011.8429531
CTCAAAT300600.011.39189151
CGCGTTT21450.011.1380811
CTCGATT69300.011.08608151
CGAATGT91750.010.9774233
CTCAGAT264750.010.7050051
CGCTATT20750.010.3850161
CTCATTT363250.010.3814431
CTCAATT185600.010.3231631
GTCGGTT74200.010.2908481
CTCGTAT92900.010.2364121
CGCATAT29400.010.1976491
CTCGGTT130000.010.0897891
ACGATAC41100.010.0504460-61
CGTATGC71650.09.804893542-43
Produced by FastQC (version 0.11.5)